Non- Tuberculosis Mycobacteria (NTM) are Mycobacteria other than Mycobacteria tuberculosis (MOTT) that are ubiquitously distributed in environment. They are also called atypical mycobacteria or anonymous mycobacteria.

They are known to cause human disease. Most commonly, NTM infects skin, soft tissue, lymph nodes, wounds, bones, joints and implant devices.

Non-tuberculous Mycobacteria [NTM]

Thermometers are used to measure temperature​. There are 4 types of thermometers used in line probe assay (LPA) laboratories:

1. Electronic Thermo Hygrometer
2. Alcohol Thermometer
3. Digital Maxima – Minima Thermometer
4. Dial Spring Thermometer

After specimen processing, the resuspended pellet may be used for various tests as per the laboratory policy or national guidelines recommended for TB diagnosis such as:

Consumables required for Truenat test provided by the manufacturer include:

Trueprep AUTO MTB Sample Pre-treatment Pack for sample processing Liquefaction buffer Lysis buffer Disposable transfer pipette (graduated) - 1ml
Trueprep AUTO v2 Universal Cartridge Based Sample Prep Kit for DNA extraction

Consumables Required at a Truenat Lab

• Use the sterile transfer pipette provided in the CBNAAT kit to draw liquefied sample into the transfer pipette
  • The minimum amount to be loaded into the cartridge is 2 ml
  • Do not process the sample if there is insufficient volume
• Open the cartridge lid
• Transfer the sample into the open port (Figure 1) of the CBNAAT cartridge and dispense slowly to minimize the risk of aerosol formation

The CBNAAT system integrates and automates sample processing with amplification and detection of the target sequences

For sample processing, sample reagent is provided in CBNAAT kit, 8ml volume pack per each cartridge

• The sample reagent solution is clear but may range from colourless to golden yellow

Processing of clinical specimens should be performed as per laboratory biosafety standards