Steps to be done after Culture Specimen Processing

After specimen processing, the resuspended pellet may be used for various tests as per the laboratory policy or national guidelines recommended for TB diagnosis such as:

• Inoculation of sediments in Mycobacterium Growth Indicator Tube (MGIT) broth (Liquid media) and or solid media for primary culture isolation
• Subculture a loopful on a Brain Heart Infusion (BHI) Agar plate for contamination check
• Concentrated smear of processed specimen can be prepared if not prepared earlier, for a microscopy smear
• DNA extraction for Line Probe Assay

Transfer the remaining sediment into 2 mL labelled cryovials and store at 2-8⁰C for repeat or additional testing.

Figure: Steps After Sample Processing

Resources​

• MGIT Procedure Manual

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