CDST_LT-M14: Line Probe Assay
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CDST_LT: Molecular basics
FullscreenUnderstanding Key elements of Molecular Biology
Structure of DNA in a Prokaryotic Cell
Central Dogma in the Function of DNA: Replication
Central Dogma in the Function of DNA: Transcription and Translation
Molecular Basis of Genetic Code and Mutation
Molecular Basis: Introduction to Polymerase Chain Reaction(PCR) Technology
Molecular Mechanism of Multi-drug Resistance in Mycobacterium tuberculosis
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CDST_LT: Introduction to LPA
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CDST_LT: LPA lab biosafety requirements
FullscreenBiosafety Requirements in Line Probe Assay [LPA] Laboratory
Biosafety Requirements in LPA Facility Design
Electrical Supply and Back-up Power Requirements in LPA Facilities
Equipment Required in Different Rooms in the LPA Lab Facility
LPA Biosafety Essentials: Procedural Biosafety Measures
LPA Biosafety Essentials: Waste Disposal
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CDST_LT: Equipment used in LPA labs
FullscreenBiosafety in TB Laboratories
Precautions for Using the Biosafety cabinet [BSC]
Biosafety Cabinet [BSC] Class I
Biosafety Cabinet [BSC] Class II Type A2
Biosafety Cabinet [BSC] Class III
Cleaning and Maintenance of the Biosafety cabinet [BSC]
Biosafety Requirements in the LPA Lab: Refrigerated Centrifuge Use
How to Use Refrigerated Centrifuges
Revolutions per minute [RPM] vs Relative centrifugal force [RCF] in the Safety Centrifuge
Protocol for Cleaning and Maintenance of Refrigerated Centrifuge
Quality Control Process of the Autoclave
Maintenance of the Autoclave in TB Labs
Common Problems and Troubleshooting Related to Autoclaves in TB Lab
LPA Laboratory Equipment: Micro Pipettes
LPA Laboratory Equipment: Hot Air Oven
LPA Laboratory Equipment: Micro Centrifuge
LPA Laboratory Equipment: Vortex Mixer
LPA Laboratory Equipment: PCR Hood
LPA Laboratory Equipment: Thermocycler
LPA Laboratory Equipment: Water Bath
LPA Laboratory Equipment: Twincubator
Laboratory Equipment: Thermometer
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CDST_LT: LPA procedures
FullscreenSpecimen Preparation for LC and LPA Labs
Extraction of DNA from Smear Positive Clinical Specimen, LJ and Liquid Media in LPA Lab Settings
Contamination Control During DNA Extraction in LPA Lab Settings
LPA PCR Reagent Preparation: Test Reagents Kit
PCR Reagent Preparation Procedure in the LPA Lab
Contamination Control During PCR Reagent Preparation in the LPA Lab
Amplification of Extracted DNA in the LPA Lab
Contamination Control in the Amplification Room in the LPA Lab
Overview of Hybridization in Line Probe Assay and Genotypic Drug Sensitivity Testing
Hybridization Setup in LPA and Genotypic DST
Hybridization Using Twincubator in LPA Lab Settings
Hybridization Using GT BLOT in LPA Lab Settings
Contamination Control in the Hybridization Area in LPA Lab Settings
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CDST_LT: LPA Recording, interpretation and reporting
FullscreenInterpretation of FL-LPA: Examples of Test Results Interpretation
Evaluation of First-line Line Probe Assay Strip
Evaluation of Second-line Line Probe Assay Strip
Interpretation of FL-LPA: Predicting TB Drug-resistance
Interpretation of FL-LPA: Predicting Drug-resistance in Special Cases
Interpretation of FL-LPA: Examples of Test Results Interpretation
Limitations of First-line Line Probe Assay [FL-LPA]
Reporting First-line Line Probe Assay [FL-LPA] Results
Reporting the Result of First-line Line Probe Assay [FL-LPA] in Special Cases
Interpretation of SL-LPA: Predicting Drug Resistance
Interpretation of SL-LPA: Predicting Drug resistance in Special Cases
Interpretation of SL-LPA: Interpretation Examples
Limitations of Second-line Line Probe Assay [SL-LPA]
Reporting Second Line-Line Probe Assay [SL-LPA] Results
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CDST_LT: LPA Troubleshooting and quality assurance
FullscreenLPA Troubleshooting for No Signal
LPA Troubleshooting for No Signal Except Conjugate Control [CC]
LPA Troubleshooting for Uneven Staining
LPA Troubleshooting for High Background Colour
LPA Troubleshooting for Other Possible Reasons
LPA Quality Assurance [QA] Including Quality Control
LPA Procedural Quality Control
LPA Quality Assurance: Proficiency Testing
LPA Quality Assurance: Cleaning of Lab