Procedure for Reading MGIT DST Results
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Procedure for Reading MGIT DST Results
Base Technical Content in text and simple images.
Principle of MGIT DST Reading
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Principle of MGIT DST Reading:
Two Mycobacterial Growth Indicator Tubes (MGITs) are inoculated with the test culture. Then, a known concentration of a test drug is added to one of the MGIT tubes (drug-containing tube), and growth is compared with the MGIT tube without the drug (growth control).
Incubation of MGIT 960 DST Sets
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Incubation of MGIT DST tubes
Inoculation of PZA Growth Control/Drug Containing Tube
Inoculation of PZA Growth Control/Drug-containing Tube
PZA Inoculum Preparation
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The PZA susceptibility test is recommended for a pure culture of the M. tuberculosis complex.
Inoculation of Growth Control Tube/Drug Containing Tubes
It is extremely important to perform quality control of drug susceptibility testing periodically.
Inoculum Preparation from MGIT Positive Culture
Inoculation Preparation for Drug-containing Tube (1-2 days)
Inoculum Preparation for First and Second-line Phenotypic DST
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Label the required number of 7 mL MGIT tubes with lab ID. In addition, label tubes with one of each of the following: GC (Growth Control), drug 1, drug 2 etc.
Preparation and Storage of Second-line Anti-TB Drugs used for DST
Preparation and Storage of Second-line Anti-TB Drugs used for DST
Critical Concentration and formulations of Second-line Anti-TB Drugs for DST
Preparation of second-line drug stock: