Pyrazinamide PZA Susceptibility Testing

The BACTEC MGIT 960 PZA medium tube contains 7 ml of broth. It consists of 5.9 g of modified Middlebrook 7H9 broth and 1.25 Casein peptone per litre of purified water adjusted to pH 5.9.

The BACTEC MGIT PZA Drug Kit contains two vials of 20,000 µg of lyophilized PZA and 6 vials of PZA supplement. Each vial of the supplement contains 15 ml of enrichment with the following formula per litre of water.

Bovine albumin---------------------------50.0 g

Dextrose -----------------------------------20.0 g

Polyoxyethyline state (POES) -----------1.1 g

Catalase -------------------------------------0.03 g

Olecic acid --------------------------------- 0.1 g

Storage instructions: PZA medium may be stored at 2-25ºC. Do not freeze, avoid exposure to light and do not use if found turbid.

Upon receipt, PZA drug vials should be stored at 2-8ºC. Once reconstituted, the antibiotic solution may be kept frozen at -20ºC or colder for up to six months but should not exceed the original expiration date. Once thawed, do not store or refreeze.

Resource

Mycobacteriology Laboratory Manual   

Assessment