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Catalase Peroxidase test for MTB

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PNB p-Nitrobenzoic acid susceptibility of MTB

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Biochemical Tests for MTB

An experienced laboratory technologist may make the presumptive diagnosis of tuberculosis on the basis of the morphological characteristics of tubercle bacilli, but it is best to do confirmatory tests.

A positive and a negative control must be tested with each new lot or a new shipment of kits received and with each new batch of extraction buffer prepared. Similarly, these controls must be run weekly or along with each batch of patient isolates when tests are set up less frequently.

1. Frequency:

The SD Bioline is a solid-phase immunochromatographic assay that is inexpensive, easy to use and readily available. They are easily stored at room temperature and allow the results within 15 -20 minutes.

Specimen preparation and subsequent steps must be performed in a BSC using full PPE. 

From positive MGIT tubes:

This method detects the MPT64 antigen specifically produced by the M. tuberculosis complex.

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