A positive and a negative control must be tested with each new lot or a new shipment of kits received and with each new batch of extraction buffer prepared. Similarly, these controls must be run weekly or along with each batch of patient isolates when tests are set up less frequently.
1. Frequency:
The SD Bioline is a solid-phase immunochromatographic assay that is inexpensive, easy to use and readily available. They are easily stored at room temperature and allow the results within 15 -20 minutes.
Specimen preparation and subsequent steps must be performed in a BSC using full PPE.
From positive MGIT tubes:
This method detects the MPT64 antigen specifically produced by the M. tuberculosis complex.
Once an MGIT tube is positive by fluorescence or visual observation, prepare a smear and stain with carbol fuchsin stain.
Procedure:
In liquid medium, M.
The following observations can make tentative identification:
The beauty of the MGIT 960 tubes is that they can be read by the instrument and also manually. There are times AFB cultures are incubated at temperatures other than 37oC. If that is the case, the MGIT tube can be incubated at an alternate temperature. These tubes would then need to be read manually with a Wood’s Lamp or other UV sources of the proper wavelength and intensity.
The MGIT 960 offers a variety of reports which may be helpful in your daily workflow. Examples of these reports can be found in your binder.