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  • Biosafety measures required for Truenat

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    General biosafety requirements for Truenat laboratory include:

     

    • Using gloves, laboratory coat, mask when handling specimens
    • Minimizing aerosol generation during sample processing and handling specimens carefully
    • Working in a well-ventilated room
    • Using laboratory work instructions, job aids, Standard Operating Procedures (SOPs) and checklists, from sample collection to reporting results, to avoid mishaps
    • Maintaining a spill management kit
    • Following manufacturer instructions to operate the instruments

     

    Precautions during processing the specimen

     

    • Handle all specimens as potentially infectious.
    • Avoid cross-contamination.
    • Nozzle of the liquefaction buffer bottle should not touch the sample container.
    • Avoid aerosol formation during the transfer of the liquefied sample to the lysis buffer bottle.
    • Use the transfer pipettes provided with the pre-treatment pack.
    • Dispose the used transfer pipettes (1% sodium hypochlorite).
    • Clean the spills with 1% sodium hypochlorite, followed by 70% alcohol.

     

    Precautions during cartridge loading and transportation

     

    • Keep the cartridges on the provided holder while loading the specimen.
    • Load the cartridges onto the machine with care.
    • Avoid turning the cartridge during transportation to prevent specimen spill.
    • If a spill occurs, soak tray in 1% sodium hypochlorite solution for 30 minutes and dispose. Spray the cartridge holder with 70% alcohol and load a new tray in the cartridge holder after 5 minutes.
    • Pipette carefully to avoid cross-contamination between reagents and/ or samples.

     

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  • Disposal of Infectious Samples and Used Cartridges in TrueNAT Laboratories

    Content

    Disposal of infectious samples and used cartridges in Truenat laboratories is an essential activity which needs to be performed to keep everyone safe and prevent TB infection in healthcare workers.

     

    • Biomedical Waste (BMW) management guidelines need to be followed-up to dispose infectious samples and used Truenat consumables.
    • Solutions and/ or solid waste containing biological samples should be disinfected before discarding.
    • Lab personnel should soak material to be disposed in freshly prepared 1% sodium hypochlorite for 30 minutes and discard.
    • Used Truenat chips, microtube, microtube cap, transfer pipette, pipette tips, reagent bottles need to be submerged in freshly prepared 1% sodium hypochlorite solution for at least 30 minutes before disposal as per biomedical waste disposal guidelines.
    • Consumables submerged in sodium hypochlorite should NOT be autoclaved,
    • Cartridge pouches, chip pouches, transfer pipette wrappers, desiccant pouches, sleeves needs to be discarded as general waste.

     

    Resources

     

     

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