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  • Trueprep Error Classes and actions

    Content

     

    Trueprep Error Classes

    The Trueprep errors are classified as:

    1. Cartridge Errors

    2. Reagent Pack Errors

    3. Device Errors and Warnings

    Trueprep error classes

    Figure 1: Classification of Trueprep errors

     

    Trueprep Error Actions

    I Cartridge Error Actions

    Trueprep error 1 actions

    Figure 2: Actions for Trueprep cartridge errors

     

    II Reagent Error Actions

    Trueprep error 2 actions

     

    Figure 3: Actions for Trueprep reagent errors

     

    III Device Error and Warning Actions

    Trueprep error 3 actions

    Figure 4: Actions for Trueprep device errors and warnings

     

    Resource

     

    Assessment

    Question    

    Answer 1    

    Answer 2    

    Answer 3    

    Answer 4    

    Correct answer    

    Correct explanation    

    Page id    

    Part of Pre-test    

    Part of Post-test    

    In ‘E11 – RTD L Error’, what does L stand for?

    Liquid

    Lower

    Light

    Lysis

        4

    In E11 - RTD L Error (The code L is for error in the Lysis Heater)

        

       Yes

     Yes

     

     

  • Truelab Error Classes and actions

    Content

    Here we discuss the error classes and actions in Truelab.

     

    Truelab Errors - Classes and Actions

    Error Class

    Error Name

    Reason for the error

    Error Actions

    Error 1

    Thermal Cycling Error

    Occurs when the test chip is faulty and the thermal cycling does not happen.

    Re-run the same elute using another chip. If the error repeats, process the sample again and re-run the elute using another chip. If the problem still persists, contact the Molbio support team.

    Error 2

    Test Stopped Manually

    Occurs when the user has manually stopped the ongoing test and the analyser does not have sufficient run time to compute data.

    Error 3

    Incorrect Optical Profile

    Occurs when there is a deviation in the expected optical profile due to a reduction in reaction volume in the chip during the course of the reaction.

    Error 4

    Runtime Error

    Occurs when run data capture/ analysis is incomplete.

    Error 5

    Probe Check Error

    Occurs in the event of a low initial signal due to insufficient mastermix dispensed onto the chip.

    Invalid

    		  

    Whenever Truelab displays the result as 'INVALID', it means that the internal control did not amplify in Polymerase Chain Reaction (PCR) or sample extraction was not proper.

     

    Resources

     

    Assessment

    Question    

    Answer 1    

    Answer 2    

    Answer 3    

    Answer 4    

    Correct answer    

    Correct explanation    

    Page id    

    Part of Pre-test    

    Part of Post-test    

    When does thermal cycling error occur in Truelab's real-time micro PCR analyzer?

    Occurs when the test chip is faulty and the thermal cycling does not happen.

    Occurs when run data capture/ analysis is incomplete.

    Occurs in the event of low initial signal.

    Occurs when the user has manually stopped the ongoing test.

        1

    Thermal cycling error occurs when the test chip is faulty and the thermal cycling does not happen.

        

       Yes

     Yes
    When does the Runtime Error occur in Truelab's real-time micro PCR analyzer?

    Occurs when the test chip is faulty and the thermal cycling does not happen.

    Occurs when run data capture/ analysis is incomplete.

    Occurs in the event of low initial signal.

    Occurs when the user has manually stopped the ongoing test.

    		       2 Runtime error occurs when run data capture/ analysis is incomplete.  

     

    Yes

     

    Yes

  • Troubleshooting Failures Without Error Codes

    Content

    The Truenat is a rapid molecular diagnostic method based on the Nucleic Acid Amplification Testing (NAAT) principle. Truenat uses portable, battery-operated devices to rapidly detect Mycobacterium tuberculosis Complex Bacteria (MTBC) and Rifampicin resistance.

    Truenat system involves two main devices:

    1. Trueprep® AUTO v2 Universal Cartridge based Sample Prep Device: Used for the automated extraction and purification of DeoxyriboNucleic Acid (DNA).
    2. Truelab® Real Time micro-PCR Analyzer: Used for performing Real-Time Polymerase Chain Reaction (RT-PCR), resulting in the semi-quantitative detection of MTBC.

    The errors in Truenat are broadly categorised as:

    • Errors with codes (Trueprep and Truelab errors)
    • Errors without codes (System failures)

    The errors without codes in Truenat and the ways to resolve them are listed in the table below:

    Sl. No. System failures Reason Troubleshoot
    1 ‘Unable to read chip information’ Analyzer was unable to read chip memory Check if the chip was loaded properly into the tray. Remove the chip and re-select the profile from Status Screen and repeat the steps. If the message reappears, load a new chip and re-load the elute again
    2 ‘Could not initialize. Please try again’ The system was unable to establish an internal connection Attempt the test again by using a new chip and re-loading the elute again
    3 “Chip is already used” OR “Chip loaded is expired” User loaded a used chip/expired chip in the tray Use a fresh chip and re-load the elute
    4 Login attempt failure Login failed due to incorrect password entered Re-enter the correct password
    5 Invalid patient name in sample details screen The patient’s name in the sample details section is blank Fill patient’s name in the sample details section

     

    Resource

     

    Assessment

     

    Question    

    Answer 1    

    Answer 2    

    Answer 3    

    Answer 4    

    Correct answer    

    Correct explanation    

    Page id    

    Part of Pre-test    

    Part of Post-test    

    What should you do when you get a system failure alert as: 

    “ Chip is already used” ?

    Re-insert the same chip

    Insert a fresh chip

    Do not insert any chip

    None of the above

        2

    When you get a system failure alert as: “ Chip is already used”  , Use a fresh chip and re-load the elute.

        

       Yes

     Yes

    What should you do when you get a system failure alert as:

    "Unable to read chip information"

    Re-insert the same chip

    Insert a fresh chip

    Do not insert any chip

    Check if chip is loaded properly, remove the chip and reselect the profile from status screen and repeat the steps. 

    		 4 When you get a system failure alert as "Unable to read chip information" Check if the chip was loaded properly into the tray. Remove the chip and re-select the profile from Status Screen and repeat the steps. If the message reappears, load a new chip and re-load the elute again  

     

    Yes

     

    Yes

     

     

  • Reset Procedure for Truenat Testing

    Content

     

    After completion of the set number of nucleic acid extractions (i.e., buffer count 05/ 25/ 50/ 100 completed) the Trueprep AUTO v2 device will prompt the alert on its 2- line LCD screen to “Change the reagent pack” and “Insert Reagent Reset Card & Start” on the screen alternatively. When this happens, the new reagent pack needs to be inserted into the device and the buffer count needs to be reset.

     

    Retest procedure for Truenat testing

    Flowchart: Procedure for Reset in Truenat Testing

     

     

    Reset procedures in Truenat testing

     

    Figure: 1) The Trueprep AUTO v2 Back Panel, with tubing and caps for plugging in reagent bottles 2) The Trueprep AUTO v2 device Back Panel with Reagent Pack 3) Reagent Reset Card; Source:Trueprep AUTO v2 Universal Cartridge Based Sample Prep Device User Manual.

     

    Resources

    Assessment

    Question     Answer 1     Answer 2     Answer 3     Answer 4     Correct answer     Correct explanation     Page id     Part of Pre-test     Part of Post-test    
    The buffer count in the Trueprep Auto V2 device will be set to what after the reset procedure is completed? 0 1 10 100 1 The buffer count will be reset to ‘0’ once the reset procedure is completed.         Yes  Yes