CBNAAT Cartridge Loading

• Use the sterile transfer pipette provided in the CBNAAT kit to draw liquefied sample into the transfer pipette
  • The minimum amount to be loaded into the cartridge is 2 ml
  • Do not process the sample if there is insufficient volume
• Open the cartridge lid
• Transfer the sample into the open port (Figure 1) of the CBNAAT cartridge and dispense slowly to minimize the risk of aerosol formation

Figure 1: CBNAAT cartridge (top view) to show the open port
(SOP for GeneXpert MTB/RIF)

• Discard the transfer pipette
• Snap the lid shut to close firmly
• Turn on the CBNAAT instrument
• Open the instrument module door, which displays the blinking green light
• Load the cartridge and close the door of the module firmly
• The test should be started within 30 minutes of adding liquefied sample to the cartridge
• The remaining liquefied sample may be kept for up to 12 hours at 2-8°C (for repeat testing)

Video 1: CBNAAT loading

Video 2: Sample Loading (Demonstration)

Resources

• SOP for GeneXpert MTB/RIF